Copenhagen 2016 Registration Programme Exhibitor Information Virtual Exhibition Satellite Meetings Glaucoma Day 2016 Hotel Star Alliance
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10 - 14 Sept. 2016, Bella Center, Copenhagen, Denmark

This Meeting has been awarded 27 CME credits

 

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Pharmacologically loaded biomatrices spray-coated on intraocular lenses as drug carriers in prophylaxis of posterior capsule opacification

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Session Details

Session Title: Presented Poster Session: PCO

Session Date/Time: Sunday 11/09/2016 | 09:30-11:00

Paper Time: 10:30

Venue: Poster Village: Pod 1

First Author: : C.Wertheimer GERMANY

Co Author(s): :    S. Kassumeh   A. Wolf   C. Priglinger   S. Priglinger   K. Eibl-Lindner  

Abstract Details

Purpose:

Since posterior capsule opacification (PCO) still represents the main longterm complication of cataract surgery, methods of pharmacologic prophylaxis are under way. One promising candidate drug might be Methotrexate The aim of this study was to determine the loading porperties of Methotrexate to the IOL with the linker molecule PLGA. Polylactic-co-glycolic acid (PLGA) is a biodegradable copolymer used in FDA approved therapeutic devices, for example OZURDEX. Therefore, we evaluated the impact of methotrexate loaded biomatrices sprayed on IOLs on PCO development in vitro.

Setting:

Laboratory for Molecular Cell Biology, Department of Ophthalmology, Ludwig Maximilian University, Munich, Germany

Methods:

Hydrophilic and hyrophobic acrylic IOLs were sprayed with methotrexate loaded PLGA. The spraying was conducted with a high influence spraying nozzle. Unsprayed, carrier only and carrier-PLGA sprayed IOLs served as controls. Simulating the human capsular bag, IOLs were set on a cell culture insert and cells were added on the edges of the IOLs. Migration and proliferation of lens epithelial cells between the membrane and the IOL towards the centre were quantified by staining the membranes with crystal violet. Toxicity of the IOLs was tested on four different cell lines from posterior and anterior segment.

Results:

Methotrexate-IOLs showed a significant difference in cell proliferation and migration compared to all controls (p < 0.001). Lens epithelial cell growth decreased massively. Especially hydrophilic acrylic IOLs, loaded with methotrexate, illustrated a strong decrease of cellular proliferation and migration. No toxicity was observed on four cell lines of the anterior and posterior segment.

Conclusions:

Coating IOLs by spraying with methotrexate loaded biomatrices using a high influence spraying nozzle seems to be a new promising method in IOL modification. PLGA as biomatrix in lens coating may become of further clinical interest due to the approved biocompatibility and the pre-existing application in clinical ophthalmology.

Financial Disclosure:

NONE

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