Copenhagen 2016 Registration Programme Exhibitor Information Virtual Exhibition Satellite Meetings Glaucoma Day 2016 Hotel Star Alliance
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10 - 14 Sept. 2016, Bella Center, Copenhagen, Denmark

This Meeting has been awarded 27 CME credits

 

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Pharmacologically pre-soaked intraocular lens as a drug delivery system in posterior capsule opacification prophylaxis

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Session Details

Session Title: Presented Poster Session: PCO

Session Date/Time: Sunday 11/09/2016 | 09:30-11:00

Paper Time: 10:20

Venue: Poster Village: Pod 1

First Author: : S.Kassumeh GERMANY

Co Author(s): :    C. Wertheimer   A. Wolf   C. Priglinger   S. Priglinger   K. Eibl-Lindner  

Abstract Details

Purpose:

Posterior capsule opacification (PCO) occurs as a common complication after cataract surgery. Disulfirame and methotrexate were previously described as potential pharmacological agents for PCO prophylaxis . The aim of our study was to evaluate the effect of methotrexate and disulfirame pre-soaked intraocular lenses (IOL) on growth of human lens epithelial cells in an in-vitro anterior segment model.

Setting:

Laboratory for Molecular Cell Biology , Department of Ophthalmology, Ludwig Maximilian University, Munich, Germany

Methods:

Hydrophilic and hydrophobic acrylic IOLs were pre-soaked in methotrexate or disulfirame. PBS-soaked IOLs served as controls. Each IOL was placed in a cell culture insert and the contact between the IOLs and the insert membrane was maintained. Human lens epithelial cells (FHL124) were added to the edges of the IOLs. The anterior segment model demonstrates cell migration and proliferation between the IOL and the membrane towards the centre and thus simulates circumstances in the human capsular bag. After incubation, inserted membranes were stained with crystal violet, to quantify actual cell proliferation and migration.

Results:

Methotrexate soaked IOLs decreased proliferation and migration of human lens epithelial cells significantly compared to the PBS-soaked control (p < 0.001). Hydrophilic and hydrophobic acrylic IOLs pre-soaked with methotrexate did not differ significantly. Even though disulfirame seemd to have potential as a drug for pharmacologic PCO prophylaxis, it was not suitable for IOL loading as it did not show any significant effect on lens epithelial cell proliferation and migration in the in-vitro anterior segment model.

Conclusions:

Methotrexate loaded IOLs may become of clinical relevance in PCO prophylaxis as it has minimized cell proliferation and migration in the in-vitro anterior segment model.

Financial Disclosure:

NONE

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