Copenhagen 2016 Registration Programme Exhibitor Information Virtual Exhibition Satellite Meetings Glaucoma Day 2016 Hotel Star Alliance

10 - 14 Sept. 2016, Bella Center, Copenhagen, Denmark

This Meeting has been awarded 27 CME credits


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The comparison of early amniotic membrane implantation and collagen cross-linking treatment’s effect on inflammation, neovascularization and ulceration mechanisms with experimental model of alkali burn

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Session Details

Session Title: Presented Poster Session: Surgical Cornea

Session Date/Time: Saturday 10/09/2016 | 15:00-16:30

Paper Time: 15:50

Venue: Poster Village: Pod 3

First Author: : S.Subasi TURKEY

Co Author(s): :    S. Subasi   O. Altintas   M. Yardimoglu   Y. Yazir   S. Furat Rencber   K. Kavram

Abstract Details


We aimed to show the effect of Crosslinking(CXL) treatment on inflammation, neovascularization and ulceration mechanisms related the acute alkali burn and to comparison with early amniotic membran implantation(AMI).


Experimental animal study


The right corneas of twenty two New Zelland rabbits were burned using a 8mm Wathman filter paper with 1N solution of NaOH and the animals were divided into four groups. Control group submitted to clinical treatment alone, an AMI group, a CXL group and an AMI +CXL group that were treated 1 h after injury. All groups were followed by the same clinical treatment . Clinical parameters ; epithelial injury, opacity, ulceration and neovascularization were evaluated post-injury at 1, 7, 14, and 18 days by two independent observers. The corneas were excised in 21st day and examined histologically and immunohistochemically.


The best results in terms of epithelial injury were in the CXL group (p=0.013). There were no statistically significant differences between study groups regarding to stromal edema, opacity and neovascularization (p=0.183,0.303 and 0.053 respectively). In evaluation of ulceration, there was no ulcerated cornea in the CXL group. In the evaluation of inflammation, a macrophage marker (CD68) was used . CD68 was significantly lower in CXL and CXL+AMI group when compared to control group, (p = 0.001 and p = 0.039, respectively). Stromal TUNEL-positive cells index which are indicators of apoptosis were significantly lower in CXL group when compared to control group (p=0.013).


CXL treatment seems effective for reepithelialization, reducing inflammation and neovascularization, and preventing ulceration in acute alkali burn treatment. The implantation of amniotic membrane after supressing of acute ocular surface inflammation is thought to be more effective in treatment of acute alkali burn. There is need for further studies on the optimal timing of the implantation of amniotic membrane

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